Product Introduction

This product consists of 3 predyed polypeptides and 2 low molecular weight proteins with a molecular weight range of 3.3 kd-31.0kd. It can be used to directly observe the protein electrophoresis status and clearly judge the membrane transfer effect of Western Blot. 5 clear blue protein bands can be seen during Tricine-glycerol SDS-PAGE gel electrophoresis and when transferred to PVDF or NC film. This product is a freeze-dried powder of protein and polypeptide mixture, each predyed protein content of about 40μg, with a 1× loading buffer.

Instructions for use:

1. After opening, it is dissolved in 200μ11 × sample buffer, and can be divided into small quantities according to needs, 10 μl per tube, stored at -20℃, and used one tube at a time to avoid repeated freezing and thawing.

2. Before use, take the packaged tubular and melt at room temperature, and then sample electrophoresis.

Schematic diagram:

< img SRC = "/ images/upload/images / 20160526/20160526164800 _73556. PNG" Alt = "gel electrophoresis map" width = "248" height = "177" title=" Gel Electrophoresis Diagram "/>

< img SRC = "/ images/upload/images / 20160526/20160526164812 _47748. PNG" Alt = "gel configuration data" width = "659" height = "289" title = "gel configuration data" / >

Gel preparation and dyeing Precautions:

1. The separation glue is prepared first, and then the sandwich glue is prepared after polymerization, and the concentrated glue is prepared finally. The colloidal product ratio of the three kinds of glue is 4:1.5:1. After 1-2h run at 30V, when the indicated front reaches the upper edge of the separation glue, the voltage is adjusted to 100V, until the end of the electrophoresis, the whole electrophoresis process takes about 6-8h.

2. After electrophoresis, the glue can be fixed in the fixing solution for 20min, and then dyed to obtain better protein bands; If time does not permit, do not fix direct dyeing. If dyeing with formula 7 is not effective or toxicity is considered, please choose our Coomas Bright blue protein glue Quick dyeing solution (Item No. :P1300), which is a fast, non-toxic and highly sensitive alternative to conventional dyeing solutions.

3. Due to the small number of amino acids contained in the polypeptide, if the polypeptide contains too many polar amino acids (basic or acidic), it will affect its band mobility on the SDS-PAGE chart, that is, its apparent molecular weight may be a certain distance from the amino acid theoretical molecular weight of the peptide.

4. Since the SDS-PAGE map shows that the molecular weight range of protein log-molecular weight and mobility is directly proportional to the linear relationship between them is 15,000-200,000, the molecular weight of proteins or peptides with molecular weight less than 10,000 can only be estimated according to the standard molecular weight to infer whether they fall into the predicted molecular weight range.

5. Due to the ultra-low molecular weight polypeptide (3000 and below), it is easy to leach from the gel, so the dyeing and decolorization time should not be too long, and the gel should not be soaked in water and preserved for too long after decolorization, otherwise the strip will disappear.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.