When some tissues contain bone or calcification, the calcium-containing tissues should not be directly embedded with paraffin. This is because of the difference in density between calcium and paraffin wax, which makes it more difficult to remove a complete section. It is best to fix the calcium-containing tissue before decalcification or both. Then downstream experiments, such as dehydration, transparency, wax dipping, embedding, and slicing, are carried out. There are many reagents used for decalcification, including organic acids, inorganic acids, ethylenediamine tetraacetic acid (EDTA) and electrolytic decalcification.

EDTA is a relatively good chelating decalcification agent, which has minimal effect on tissue structure and can preserve some enzymes of tissues well. The tissues decalcified by EDTA can be stained by immunohistochemistry and in situ hybridization. However, the decalcification rate is too slow, generally taking weeks to months.

The advantages of Solarbio EDTA decalcification solution are as follows: (1) The results of tissue staining by EDTA decalcification are good; ② Little damage to the structure of the tissue; ③ The end point of decalcification can be determined by chemical tests.

Disadvantages of Solarbio EDTA decalcification solution: (1) The decalcification rate is very slow, and it is not suitable for decalcification of conventional specimens; ② The tissue will harden slightly after decalcification.

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