PonceauSSolution,10×
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Cat.No：P0012 Solarbio
Storage：Store at RT，1 year
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Product Information
| Name | PonceauSSolution，10× |
| Storage | Store at RT，1 year |
| Unit | Bottle |
| Specification | 10ml |
Ponceau S, also known as scarlet, can be used for the detection of proteins on PVDF membranes, nitrocellulose membranes and cellulose acetate membranes. Ponceau is negatively charged and can bind to positively charged amino acid residues, while ponceau can also bind to non-polar regions of proteins to form red bands. The sensitivity of ponceau dyeing is less than that of Coomassie brilliant blue dyeing. The staining of proteins by ponceau is reversible and can be washed away with distilled water, PBS, or other appropriate solutions. Therefore, during protein N-terminal sequencing, the protein on SDS-PAGE glue was transferred to PVDF membrane, and the target strip was cut off for sequencing after staining with ponceumin.
Note: Ponceau staining solution is not suitable for the detection of proteins on nylon membranes. Ponceau dyeing solution can also be directly used to dye the PAGE glue, but it is best to soak the PAGE glue with methanol-acetic acid solution before dyeing to play a role in fixing the protein.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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