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Determination of Significance：
Glutamine is the amide of glutamic acid and one of the important amino acids that make up proteins. At the same time, glutamine is also the main source of α-ketoglutaric acid in the tricarboxylic acid cycle. Glutamine exists in the organism in two states: free state and bound state. Free glutamine plays an important role in organism metabolism, and its metabolism accounts for more than 60% of free amino acids in cells and blood circulation.
Measurement Principle：
Free glutamine is converted to glutamic acid under the catalysis of glutaminase, Under the catalysis of glutamate dehydrogenase (GDH), glutamate and NAD are produced α- Ketoglutaric acid, NADH and NH4+, under the action of 1-mPMS, WST can react with NADH to produce water-soluble formazan, which has the maximum absorption peak at 450 nm, and the glutamine content can be calculated.
Self Provided：
Microplate Reader/Spectrophotometer, Desktop Centrifuge, Water Bath/Constant Temperature Incubator, Transferpettor, Mortar/Homogenizer/Cell Ultrasonic Crusher, Micro Glass Cuvette/96 Well Flat-bottom Plate, Chloroform and Distilled Water.
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