Storage:Store at -20℃,6 months
Determination of Significance:
Pyrroline-5-carboxylate reductase (P5CR) is an important housekeeping protein widely found in prokaryotes and eukaryotes. In the presence of NAD(P)H, pyrroline-5-carboxylic acid (P5C) is converted to proline by pyrroline-5-carboxylate reductase, and P5CR has also been found to be involved in the metabolism of thioproline in E. coli.
Measurement Principle:
Thioproline is dehydrogenated by pyrroline-5-carboxylate reductase and is accompanied by the conversion of NAD to NADH. In the presence of 1-mPMS, WST-1 reacts with NADH to produce water-soluble formazan with a characteristic absorption peak at 450 nm.
Self Provided:
Spectrophotometer, water bath/constant incubator, adjustable pipette, analytical balance, mortar/homogenizer/cell sonicator , micro glass cuvettes/96 well plates, distilled water and ice.
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