Determination of Significance:
Sialic acid (SA), also known as N-acetylneuraminic acid, is an important component of glycoproteins and glycolipids on the cell membrane. It widely exists in organisms and participates in various physiological functions on the cell surface.
Measurement Principle:
Sialic acid forms a purplish red complex with 5-methylresorcinol in the presence of oxidant. It has a maximum absorption peak at 560nm. The content of sialic acid can be calculated by measuring the light absorption value of the product at 560nm.
Self Provided:
Spectrophotometer/Microplate reader, centrifuge, water bath/constant temperature incubator, transferpettor, mortar/homogenizer/cell ultrasonic crusher, micro quartz cuvette/96 well UV flat-bottom plate, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.