Product Introduction

In the process of constant temperature amplification of nucleic acid molecules, whether LAMP, CPA and other amplification techniques are used in the process of constant temperature amplification of nucleic acid molecules, such high speed amplification characteristics will inevitably lead to false positive amplification. The amplification of false positives is particularly obvious in LAMP amplification. Due to the high concentration of primers used in LAMP amplification, a small amount of mismatches and primer Dimer are inevitably guaranteed, and these slight contamination will lead to false positives in LAMP high-speed amplification. This product is optimized reaction buffer, combined with unique color development technology and negative control technology to establish the premix constant temperature mix series of products, which can be used for LAMP red and yellow color change, LAMP purple and green color change, LAMP orange and green (OG) color change with high sensitivity and high specificity. In LAMP amplification experiments, the Bst 4.0 MasterMix has faster amplification speed and impurity tolerance, thanks to the excellent performance of Bst 4.0 DNA/RNA polymerase, compared with the DNA/RNA LAMP MasterMix or LAMP Mix provided by other companies.

Bst 4.0 DNA/RNA polymerase has RNA template-dependent polymerase activity (reverse transcription) and DNA-dependent polymerase activity, so both DNA and RNA samples can be amplified using the product for thermostatic amplification.

As LAMP produces a large amount of H+ during the reaction, resulting in a decrease in pH of the reaction system (usually from pH8.8 to below 7.2), therefore, under the condition of low buffer salt system, PH-sensitive dyes can be used to achieve visual LAMP detection. The product contains a Tris (pH8.8) buffer salt with a final concentration of 25 μM. Color-changing LAMP amplification can be performed with a pH sensitive dye. In addition, the reagent does not contain glycerol and can be used to establish a freeze-drying system.

Precautions

(1) The reagent is sensitive to buffer salts that affect pH, so components such as Tris salt and NaOH in the template have a crucial effect on the reaction. Elution with ddH2O is recommended when nucleic acid purified samples are tested.

(2) It is recommended to use samples stored by ddH2O for testing when using crude samples. When the crude sample is tested, the best crude sample is the swab sample. After the swab sample is soaked in ddH2O, the swab sample can be amplified directly using the soaking solution as a template without the need for nucleic acid purification.

(3) Special note on the use of DEPC water: Because DEPC treated ddH2O shows strong acidity, it will directly cause the lyophilized ball to become yellow after dissolution. Therefore, DEPC treated H2O must be adjusted by NaOH solution pH to 8.0-9.0 before use. We recommend direct use of 18.2ΩH2O prepared by the water cooler without affecting the amplification of the RNA sample.

(4) The reagent does not contain glycerin components, and personnel with experience in freeze-drying may develop freeze-drying reagents.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.