Product description:

Primary cell small nucleic acid transfection reagent is a reagent specially used for primary cell small nucleic acid transfection, which is improved and developed on the basis of cell line small nucleic acid transfection reagent. The product can be used to transfect siRNA, miRNA mimics and miRNA inhibitor, etc. This product can efficiently transfect the vast majority of primary cells and obtain ideal gene knockout effect. Even for some living parasite larvae, ideal transfection results can be obtained. For most primary cells, the positive rate of cell transfection was more than 80%, while the death rate of transfected cells was less than 10%. It is also extremely easy to use, first mix sRNA with RFect at room temperature, and then add the mixture of siRNA and transfection reagent directly to the cells containing culture medium. The serum has no effect on the transfection effect, and there is no need to deliberately add or change the culture medium.

Product characteristics:

The transfection performance of primary cells: the positive rate of cell transfection is generally more than 80%, the effect of gene knockout is obvious, and the cytotoxicity of hepatocytes with Lamin A/C gene knockout efficiency is more than 95%.

Extremely low cytotoxicity: the death rate of transfected cells is less than 10%, which greatly reduces the effect of cytotoxicity on the experimental results.

A wide range of transfected cells: the vast majority of primary cells can obtain ideal transfection results.

Suitable cells:

Clone9, HUVEC, Primary Human Fibroblasts, MSC, BE(2)C, MRC-5, NIH3T3, B16F10, BNL.CL2, C6 and so on.

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