Efficient Primary Cell Nucleic Acid Transfection Kit (For serum-containing medium)

Product description:

Small nucleic acid transfection reagent is a new type of small nucleic acid transfection reagent. The product can be used to transfect small molecular RNA within 200 bp, such as siRNA, miRNA mimics, miRNA inhibitor, etc. The transfected cells include most adherent cells, such as general cell lines, tumor cell lines and so on. At present, no matter at home or abroad, the main components of transfection reagents are liposomes or polyethyleneimine (Polyethylenimine, PEI). These two components have great cytotoxicity, and the transfection effect is not good. The product uses a new type of animal-derived nanomaterials with low toxicity and high transfection performance. Compared with other brands of small nucleic acid transfection reagents, the cell transfection positive rate of this product is generally more than 90%, and the inhibition efficiency of Lamin A/C gene is more than 95%, while the cell transfection positive rate of other brand transfection reagents is generally no more than 70%. The cytotoxicity is very low, and the death rate of transfected cells is less than 10%, while that of other brand reagents is generally more than 30%. The serum has no effect on the transfection effect, and it is not necessary to add or replace the culture medium deliberately. This product is suitable for cell line transfection and primary cell transfection, please choose small nucleic acid transfection reagent.

Product characteristics:

Cell transfection performance: cell transfection positive rate is more than 90%, gene knockout effect is obvious, A549 cell Lamin A/C gene knockout efficiency is more than 95%;

Extremely low cytotoxicity: transfection cell mortality is less than 10%, greatly reducing the effect of cytotoxicity on experimental results;

A wide range of transfected cells: the vast majority of adherent cell lines can obtain ideal transfection results.

Suitable cells:

293T, A549, HEK293, HeLa, MCF-7, HepG2, HT-1080, HLF-a, Huh-7, KB, PC3, BRL-3A, DU145, Hepa1-6, OVCAR3, NCaP-FGC, C2C12, MDCK, CHO-K1, islk.219, HT-29, NCI-H1688, RKO, Vero, COS-1, COS7-L, COS-7, SK-BR3, Hepa1cLc7, Daoy, DBTRG-05MG, KLN205, Neuro-2a, DI-TNC1 and so on.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.