Active Protein Extraction Kit provides a complete set of reagents suitable for extracting active protein from a variety of primary or subcultured animal cells and a variety of animal solid tissue samples such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue, etc. When combined with other reagents, it can also be used for the extraction of active protein from plants, bacteria, fungi, yeast and other samples. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation, which has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lyse time needs to be optimized according to the actual sample situation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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