Cytoplasmic Protein/Nucleoprotein/Histone Extraction Kit
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Cat.No：EX1580 Solarbio
Storage：Store at 2-8℃，1 year
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Endocrinology & Hormones
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Hippo
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Protein and Immunity > Protein Extraction/Quantification > Protein Extraction Kit > Cytoplasmic Protein/Nucleoprotein/Histone Extraction Kit
Product Information
| Storage | Store at 2-8℃，1 year |
| Unit | Kit |
| Specification | 50T 100T |
Cytoplasmic protein/nucleoprotein/histone extraction kit provides a complete set of reagents suitable for the extraction of cytoplasmic protein/nucleoprotein/histone from a variety of primary or pass-culture animal cells and a variety of animal solid tissue samples. This kit contains a unique formulation that effectively dissolves cell membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation, which has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lyse time needs to be optimized according to the actual sample situation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Stiff substrate induces nucleus pulposus cell ferroptosis via YAP and N-cadherin mediated mechanotransduction
Click to check >>Author：Wencan Ke, Zhiwei Liao, Huaizhen Liang, Bide Tong, Yu Song, Gaocai Li, Liang Ma, Kun Wang, Xiaobo Feng, Shuai Li, Wenbin Hua, Bingjin Wang, Cao Yang
IF：11.0920
Publish_to：Advanced Healthcare Materials
PMID：37022980
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