The total protein extraction kit of Gram-positive bacteria can extract total protein from a variety of Gram-positive bacteria, and the kit is suitable for protein extraction of a variety of Gram-positive bacteria and a variety of firmicutes, and can also be used for crude preparation of purified proteins and total protein preparation. The extraction process is simple and convenient. This kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protease from degrading the protein and provides a guarantee for extracting high-quality protein. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The proteins extracted in this kit are active proteins with a natural protein conformation that can be used for different downstream applications. Depending on different samples and different culture conditions, each ml of bacterial solution can probably extract 2-10mg of protein. The protein was quantified by BCA or Lowry method. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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