Transmembrane proteins undertake various biological functions and play an important role in the occurrence and development of diseases. The preparation of transmembrane protein samples needs to be fully considered for downstream applications such as glue analysis and mass spectrometry, so the preparation of transmembrane protein samples becomes an insurmountable challenge. The traditional method of preparing transmembrane protein samples is to use detergent and surfactant solubilization. Stain remover treatment can make transmembrane proteins lose their natural structure, thus hindering the functional study of transmembrane proteins. Plant transmembrane protein extraction kit is a high yield transmembrane protein extraction kit based on chemical method. Plant transmembrane protein extraction kit can extract total transmembrane protein from various plants, and can be used for crude preparation of purified protein and transmembrane protein preparation. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protease from degrading the protein and provides a guarantee for extracting high-quality protein. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: plants

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