Insect cell transmembrane protein extraction kit is a high yield transmembrane protein extraction kit based on chemical method. This kit provides a complete set of reagents for the gentle and efficient extraction of membrane/transmembrane proteins and membrane protein complexes from insect tissues or cultured cells. The proteins extracted and enriched with this kit range from very small molecular weights to very large proteins, from a single transmembrane to more than 7 times, and even some large protein complexes. The extraction process is simple and convenient, and can be completed within 1 hour. The extracted membrane protein not only has high purity and natural activity, but also has little cross contamination. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
Applicable sample: insects
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