Animal membrane proteins perform various biological functions and play an important role in the development of life. The preparation of membrane protein samples needs to be fully considered for downstream applications such as glue analysis and mass spectrometry, so the preparation of membrane protein samples becomes an insurmountable challenge. The traditional method of preparing membrane protein samples is solubilization with stain remover and surfactant. Stain remover treatment can make membrane proteins lose their natural structure, thus hindering the functional study of membrane proteins. Insect cell plasma membrane protein extraction kit is a high-yield membrane protein extraction kit based on chemical rather than detergent methods. This kit provides a complete set of reagents for the gentle and efficient extraction of plasma membrane proteins from insect tissues or cultured cells. The proteins extracted and enriched with this kit range from very small molecular weights to very large proteins, from a single transmembrane to more than 7 times, and even some large protein complexes. The extraction process is simple and convenient, and can be completed within 1 hour. The extracted membrane protein not only has high purity and natural activity, but also has little cross contamination. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: insects

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