Transmembrane proteins undertake various biological functions and play an important role in the occurrence and development of various life activities such as cell proliferation and differentiation, energy conversion, signal transduction and material transport. The preparation of membrane protein samples needs to be fully considered for downstream applications such as glue analysis and mass spectrometry, so the preparation of membrane protein samples becomes an insurmountable challenge. Fungal membrane protein extraction kit (Small fungus) is a fast, efficient and high-yield membrane protein extraction kit. Fungal membrane protein extraction Kit (Microfungi) can extract membrane proteins from a variety of small fungi (filamentous fungi, etc.) in addition to large fungi (fungi). The optimized formula of lysate can fully release membrane proteins from all kinds of membranous and non-membranous mycelia proteins, and can be used for crude preparation of purified proteins and membrane protein preparation. The extraction process is simple and convenient. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: fungus

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