Transmembrane proteins undertake a variety of biological functions, and the preparation of membrane protein samples needs to be fully considered for downstream gel analysis and mass spectrometry applications, so the preparation of membrane protein samples has become an insurmountable challenge. Yeast membrane protein/cytoplasmic protein extraction kit is an efficient and high-yield membrane protein extraction kit. The yeast membrane protein/cytoplasmic protein extraction kit can extract membrane protein/cytoplasmic protein from various yeast samples, and can be used for crude preparation of purified proteins and membrane protein preparation. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: Yeast

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