Escherichia coli membrane protein extraction kit is an efficient and high-yield membrane protein extraction kit. Escherichia coli membrane protein extraction kit can extract membrane protein from various Escherichia coli bodies, and can be used for crude preparation of purified protein and membrane protein preparation. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture that prevents the protease from degrading the protein, providing a guarantee for the extraction of high-quality proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation, which has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lyse time needs to be optimized according to the actual sample situation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: E. coli

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