The nuclear protein extraction kit provides a complete set of reagents for extracting nuclear and cytoplasmic proteins from a variety of primary or successive animal cells and a variety of animal solid tissues, such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue, etc. The extraction process is simple and convenient, and can be completed within 1 hour. The prepared nucleoprotein and cytoplasmic protein not only have high purity, maintain natural activity, but also have little cross contamination. This kit contains a unique formulation that effectively dissolves nuclear membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, transcriptional activity analysis, Gelshift gel block assay, immunocoprecipitation, enzyme activity determination and other protein studies. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography. The protein extracted by this kit is an active protein with natural protein conformation.

Applicable sample: animal tissue

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