Mitochondria are organelles covered by two layers of membranes in eukaryotic cells, and are important organelle structures for producing energy in cells, and are the main place for cells to perform aerobic respiration. The energy substances in the cell - fat, sugar, and some amino acids are finally oxidized here, and ATP is generated by coupling phosphorylation to supply the physiological activities of the cell. The study of mitochondrial structure and function is usually carried out on mitochondria in vitro. Plant mitochondrial protein extraction kit provides a complete set of reagents, with a simple and rapid method to extract plant mitochondrial protein within 1 hour. This kit contains a unique formulation that effectively dissolves mitochondrial membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation.
Applicable sample: plants
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