Mitochondria are important organelles that produce energy in eukaryotic cells. The energy substances in the cell - fat, sugar, and some amino acids are finally oxidized here, and ATP is generated by coupling phosphorylation to supply the physiological activities of the cell. The study of mitochondrial structure and function is usually carried out on mitochondria in vitro. Yeast mitochondrial Protein Extraction Kit provides a complete set of reagents suitable for the extraction of mitochondria from a variety of yeast samples. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation, which has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lyse time needs to be optimized according to the actual sample situation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

Applicable sample: Yeast

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