Mitochondria are important organelles to produce energy mitochondria in eukaryotic cells, and the energy substances in the cell - fat, sugar and part of amino acids - perform final oxidation here, and produce ATP by coupling phosphorylation to supply cell physiological activities. Transmembrane proteins undertake various biological functions and play an important role in various biological development processes. The preparation of membrane protein samples needs to be fully considered for downstream applications such as glue analysis and mass spectrometry, so the preparation of membrane protein samples becomes an insurmountable challenge. Plant mitochondrial membrane protein extraction kit can be used to extract mitochondrial membrane proteins from various plant samples in a simple and rapid method. The extraction process is simple and convenient, and high quality mitochondrial membrane proteins can be extracted within one hour. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protease from degrading the protein and provides a guarantee for extracting high-quality protein. This kit contains a unique formulation that effectively dissolves membrane components. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation.

Applicable sample: plants

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