Mitochondrial protein/Cytoplasmic protein Extraction Kit provides a complete set of reagents for the extraction of mitochondrial and cytoplasmic proteins from a variety of primary or transmissible cells and various solid tissues such as brain, spinal cord, nerve junctions or fibers, fat, liver, digestive tract, kidney, heart, muscle, blood vessels, connective tissue and other mammalian tissues. The extraction process is simple and convenient. The prepared mitochondrial proteins not only have high purity and natural activity, but also have little cross-contamination. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
Applicable sample: animal cells/tissues
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