The cytoplasmic protein Extraction kit offers a complete set of reagents for the preparation of cytoplasmic proteins from cells and animal tissues. The extraction process is simple and convenient, and can be completed within 1 hour. The prepared cytoplasmic protein not only has high purity and natural activity, but also has little cross contamination. All components of this kit do not contain detergent components, and the composition of the final protein sample has no influence on downstream applications such as NI column purification, molecular sieve, ion exchange, affinity purification, etc. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation, which has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lyse time needs to be optimized according to the actual sample situation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
Applicable sample: animal cells/tissues
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.