Plant Protein Extraction Kit (no detergent components) provides a complete set of reagents for extracting total protein from a variety of plant cell and tissue samples. The extraction process is simple and convenient. All components of this kit do not contain detergent components, and the composition of the final protein sample has no significant impact on downstream applications such as NI column purification, molecular sieve, ion exchange, affinity purification, etc. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
Applicable sample: plants
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