Yeast protein extraction kit is suitable for efficient and gentle extraction of soluble total protein from a variety of yeast samples. The extraction process is simple and convenient, avoiding the destruction of yeast cells by grinding method, ultrasonic method or pressing method with poor repeatability, avoiding the destruction of the target protein caused by oxidation and heat increase caused by intense mechanical treatment. All components of this kit do not contain detergent components, and the composition of the final protein sample has no influence on downstream applications such as NI column purification, molecular sieve, ion exchange, affinity purification, etc. This kit contains a unique formulation that effectively dissolves membrane components, including cytoplasmic membrane, nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation.
Applicable sample: Yeast
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