The nuclear protein extraction kit provides a complete set of reagents for extracting nuclear and cytoplasmic proteins from a variety of primary or successive animal cells and a variety of animal solid tissues, such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue, etc. The extraction process is simple and convenient, and can be completed within 1 hour. The prepared nucleoprotein and cytoplasmic protein not only have high purity, maintain natural activity, but also have little cross contamination. This kit contains a unique formulation that effectively dissolves nuclear membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect the mass spectrometry experiment. After dialysis or desalting treatment, the final protein sample will not contain detergent, high concentration salt and other effects. Basically, it can satisfy any downstream proteomic related experimental research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid the mass spectrometry peak shift caused by AEBSF, so the protein samples extracted from this product can be used for MassSpectrometry (MS) detection and analysis, proteomics and other related research. The protein extracted by this kit is an active protein with natural protein conformation.

Applicable sample: animal cells/tissues

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