Animal transmembrane proteins undertake various biological functions and play an important role in the occurrence and development of diseases. The preparation of membrane protein samples needs to be fully considered for downstream applications such as glue analysis and mass spectrometry, so the preparation of membrane protein samples becomes an insurmountable challenge. Membrane protein extraction kit is a fast, efficient and high-yield membrane protein extraction kit. This kit provides a complete set of reagents suitable for the extraction of total membrane proteins from animal cells and animal tissues. The extraction process is simple and convenient, and can be completed within 1 hour. The extracted membrane protein not only has high purity and natural activity, but also has little cross contamination. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect the mass spectrometry experiment, which can basically meet the requirements of any downstream proteomic related experimental studies. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid the mass spectrometry peak shift caused by AEBSF, so the protein samples extracted from this product can be used for MassSpectrometry (MS) detection and analysis, proteomics and other related research. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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