Plant Nucleoprotein Extraction Kit (For proteome test, mass spectrometry)-Non-enzymatic method

Plant nuclear protein extraction kit provides a complete set of reagents suitable for extracting nuclear protein from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient, and can be completed within 1 hour. The prepared nucleoprotein not only has high purity and natural activity, but also has little cross contamination. This kit contains a unique formula that effectively dissolves plant nuclear components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect the mass spectrometry experiment. After dialysis or desalting treatment, the final protein sample will not contain detergent, high concentration salt and other effects. Basically, it can satisfy any downstream proteomic related experimental research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid the mass spectrometry peak shift caused by AEBSF, so the protein samples extracted from this product can be used for MassSpectrometry (MS) detection and analysis, proteomics and other related research. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography. The protein extracted from this kit can also be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel blocking experiment, enzyme activity determination and other downstream protein research experiments.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.