Fungal protein extraction kit (suitable for proteomics experiments) is suitable for extracting total proteins from various filamentous fungi. The optimized lysate formula can fully release a variety of membrane and non-membrane mycelium proteins as well as a variety of mycelium variant proteins, and the extraction process is simple and convenient, which can be completed within 1 hour. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect the mass spectrometry experiment. After dialysis or desalting treatment, the final protein sample will not contain detergent, high concentration salt and other effects. Basically, it can satisfy any downstream proteomic related experimental research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid the mass spectrometry peak shift caused by AEBSF, so the protein samples extracted from this product can be used for MassSpectrometry (MS) detection and analysis, proteomics and other related research.

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