Plant Mitochondria Extraction Kit-Non-enzymatic method

Mitochondrial membrane potential detection kit (JC-10) is a rapid and sensitive kit for detecting the changes of mitochondrial membrane potential in cells, tissues or purified cells, tissues or purified mitochondrial membrane potential using JC-10 as a fluorescent probe. It can be used to detect early apoptosis.

JC-10 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential △Ψm . The membrane potential of cells, tissues or purified mitochondria can be detected. When the mitochondrial membrane potential is high, JC-10 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence; when the mitochondrial membrane potential is low, JC-10 can not gather in the mitochondrial matrix, when JC-10 is a monomer, it can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. The relative ratio of red and green fluorescence is often used to measure the proportion of mitochondrial depolarization.

The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition of JC-10 from red fluorescence to green fluorescence. At the same time, the transition of JC-10 from red fluorescence to green fluorescence can also be used as an early detection index of apoptosis.

The maximum excitation wavelength of JC-10 monomer is 515 nm and the maximum emission wavelength is 529 nm. The maximum excitation wavelength of JC-10 polymer is 585 nm and the maximum emission wavelength is 590 nm. In the actual observation, you can use the routine settings of observing red fluorescence and green fluorescence.

This kit provides CCCP as a positive control for inducing the decrease of mitochondrial membrane potential. For the samples in the 6-well plate, this kit can detect a total of 100 samples; for the samples in the 12-well plate, this kit can detect a total of 200 samples.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

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2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.