English
中文
CAS:11070-73-8
Storage:Powder:-20℃，1 year;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Appearance:White to off-white Solid
Manual Download
Examples of using this product（for reference only）
In Vitro:
Cell （Adipogenic differentiation；1.9 μM Insulin,2d；10 μg/mL Insulin,14d/change the medium every two days）:
The resuscitated cells were seeded in 12-well plates for culture, and the medium was changed every other day until the cell confluency reached 70?80%. For adipogenic differentiation, the differentiation medium was replaced. The differentiation medium contained 10% FBS, 1 mM 3- isobutyl-1-methylxanthine, 1 μM dexamethasone, and 1.9 μM insulin (insulin from bovine pancreas, Solarbio, China) in DMEM. This process lasted for two days, and then, the medium was changed to adipocyte maintenance medium (DMEM supplemented with 10% FBS and 10 μg/mL insulin) and cultured for 14 days (change the medium every two days). The differentiated cells were harvested and fixed for 30 min with 4%
paraformaldehyde. The fixed cells were stained with oil red O for 60 min. The stained cells were washed three times with isopropanol for further examination under an inverted fluorescence microscope. Five images of the cells after cryopreservation and differentiation of fresh cells were selected, and the differentiated and undifferentiated cells were counted and the differentiation efficiency was calculated.
Reference：
Xia Y, Huang LX, Chen H, Li J, Chen KK, Hu H, Wang FB, Ding Z, Guo SS. Acoustic Droplet Vitrification Method for High-Efficiency Preservation of Rare Cells. ACS Appl Mater Interfaces. 2021 Mar 24;13(11):12950-12959. doi: 10.1021/acsami.1c01452. Epub 2021 Mar 11. PMID: 33703892.
