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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-β-Actin Polyclonal antibodyReactivity：Mouse,Rat,Monkey,Human
Application：IF,IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：60
Host：Rabbit
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| Name | Anti-β-Actin Polyclonal antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Pig;Chicken;Horse;Rabbit;Dog;Sheep;Chimpanzee |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. IF 1:50-200. |
| Target | ACTB |
| Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Swiss Prot | P60709 |
| Gene ID | 60 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human ACTB |
| Public Immunogen Range | 1-375aa |
| Calculated MW | 41kDa |
| Observed MW | 46kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with β-actin antibody diluted at 1:2000;Lane: Caski,MCF7,HEK293,NIH3T3,PC12,COS7,A549,THP-1,293T,Mouse lung,Mouse spleen,Mouse kidney,Mouse thymus.
Immunohistochemistry of paraffin-embedded Human breast cancer with β-actin antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using β-actin antibody diluted at 1:100.
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