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Your Position: Home > Biochemical Assay Kits > Esterase Series > Fluoride Resistant Acid Phosphatase Activity Assay Kit
Fluoride Resistant Acid Phosphatase Activity Assay Kit

Storage:-20℃

  BC5450Manual
Fluoride Resistant Acid Phosphatase Activity Assay Kit
Cat.No：
BC5450
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC5450-50T/24S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

Fluoride resistant acid phosphatase (FRAP) is a type of acid phosphatase. FRAP is mainly found in the lysosomes of most cells, the prostate gland, brain, liver, spleen, and platelets.

Measurement Principle：

The activity of fluoride-resistant acid phosphatase is not inhibited by fluoride ions, whereas the activity of other acid phosphatases is inhibited by fluoride ions. Under acidic conditions, the fluoride-resistant ion acid phosphatase catalyzes the production of p-nitrophenol from PNPP. The p-nitrophenol appears yellow under alkaline conditions and can be detected at 400 nm absorbance. The darker the yellow color of the product, the higher the activity of the fluorine-resistant ion acid phosphatase, and vice versa, the lower the enzyme activity.

Self Provided：

Spectrophotometer, centrifuge, water bath/incubator, adjustable pipette, 1mL glass cuvette, mortar/homogenizer/cell sonicator, ice and distilled water

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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