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Cell Stimulation Kit（Without protein transport inhibitor)

Cat.No：IK-CEA-2 Solarbio

Storage：Store at -20℃，6 months

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Product Information

Storage	Store at -20℃，6 months
Unit	Kit
Specification	2*100ul

CAS号	产品货号	产品名称	分子式	分子量	对应靶点	产品描述
16561-29-8	IP1010	Phorbol 12-Myristate 13-Acetate；佛波酯	C36H56O8	616.83	PKC	PMA是一种佛波酯，是常用的 PKC 活化剂。
56092-82-1	II0820	Ionomycin Calcium Salt；钙离子霉素	C41H70O9Ca	747.1	calcium ionophore	是一种有效的，选择性的钙离子载体 (calcium ionophore)，也是一种抗生素。能够促进细胞凋亡，诱导蛋白激酶C (PKC) 激活。
本套装内含PMA(81μM in DMSO)、Ionomycin(1.34mM in EtOH)各一支，每支溶液100μL。在细胞内，PKC的激活可以引起下游众多蛋白激酶的磷酸化，形成级联反应，导致多种蛋白的表达，进而引起细胞的活化。PKC可以在DAG和Ca2+共同作用下被激活。PMA是一种有效的PKC激活剂；Ionomycin是一种有效的，选择性的钙离子载体。PMA常与Ionomycin联用，刺激免疫细胞活化，用于诱导体外细胞培养物产生细胞因子。常与蛋白转运抑制Kit-1（Cat:IK-PTI-1）联用。同时，也可加入蛋白转运抑制剂Monensin或Brefeldin A，抑制细胞因子外分泌，以准确分析细胞因子的表达水平。如果对分泌蛋白进行免疫检测（例如 ELISA、蛋白免疫印迹或多重蛋白检测），则无需使用Monensin或Brefeldin A对细胞进行处理。

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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