Cell Stimulation Kit(Without protein transport inhibitor)
Storage:Store at -20℃,6 months
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CAS号 | 产品货号 | 产品名称 | 分子式 | 分子量 | 对应靶点 | 产品描述 |
16561-29-8 | IP1010 | Phorbol 12-Myristate 13-Acetate;佛波酯 | C36H56O8 | 616.83 | PKC | PMA是一种佛波酯,是常用的 PKC 活化剂。 |
56092-82-1 | II0820 | Ionomycin Calcium Salt;钙离子霉素 | C41H70O9Ca | 747.1 | calcium ionophore | 是一种有效的,选择性的钙离子载体 (calcium ionophore),也是一种抗生素。能够促进细胞凋亡,诱导蛋白激酶C (PKC) 激活。 |
本套装内含PMA(81μM in DMSO)、Ionomycin(1.34mM in EtOH)各一支,每支溶液100μL。 在细胞内,PKC的激活可以引起下游众多蛋白激酶的磷酸化,形成级联反应,导致多种蛋白的表达,进而引起细胞的活化。PKC可以在DAG和Ca2+共同作用下被激活。PMA是一种有效的PKC激活剂;Ionomycin是一种有效的,选择性的钙离子载体。PMA常与Ionomycin联用,刺激免疫细胞活化,用于诱导体外细胞培养物产生细胞因子。常与蛋白转运抑制Kit-1(Cat:IK-PTI-1)联用。同时,也可加入蛋白转运抑制剂Monensin或Brefeldin A,抑制细胞因子外分泌,以准确分析细胞因子的表达水平。如果对分泌蛋白进行免疫检测(例如 ELISA、蛋白免疫印迹或多重蛋白检测),则无需使用Monensin或Brefeldin A对细胞进行处理。 |
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
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