Trypsin-EDTA 2.5%, without Phenol red

(This product is 10X mother liquor, 1X is recommended for working concentration)

In vitro culture of histiocytic cells, in primary cell culture of histiocytic dispersion (preparation of tissue blocks into a single cell suspension), and in pass-through cell culture, digestion and dispersion of adherent growth cells are performed using histiocytic digestion fluid. Commonly used digestive fluids are trypsin, EDTA, etc., whose function is mainly to hydrolyze intercellular proteins (such as extracellular matrix), disperse tissues or adherent cells into single cells, and make cell suspension for further experiments.

The Trypsin-EDTA Solution produced by Solarbio contains 2.5% trypsinase 0.2% EDTA (5.3mM), which is dissolved in calcium and magnesium free balanced salt solution, and can be directly used for digestion of cultured cells and tissues after filtration. This product has the characteristics of convenience, fast, stable and safe, good cell state, etc. Most adherent cells can be digested in about 2 minutes at room temperature. Trypsin-edta digestive solution has two kinds of products, including phenol red and no phenol red, and phenol red has the pH indicator function.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.