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Determination of Significance：
Lactic acid is an important intermediate product in the process of biological metabolism, which is closely related to glucose metabolism, lipid metabolism, protein metabolism and intracellular energy metabolism. Lactic acid content is an important index to evaluate glycogen metabolism and aerobic metabolism.
Measurement Principle：
Under the action of D-lactate dehydrogenase, pyruvate is generated from D-lactic acid, and NAD+ is reduced to produce NADH and H+. Under the action of 1-mPMS, WST-1 reacts with NADH to produce water-soluble formazan, which has the maximum absorption peak at 450nm, based on which the content of D-lactic acid can be calculated.
Self Provided：
Spectrophotometer, mortar/homogenizer/cell ultrasonic crusher, centrifuge, constant temperature foster box/water-bath, 1 mL glass cuvette, ice and distilled water.
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