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Your Position: Home > Biochemical Assay Kits > Other Series > Phosphotransacetylase（PTA）Activity Assay Kit
Phosphotransacetylase（PTA）Activity Assay Kit

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  BC5460Manual
Phosphotransacetylase（PTA）Activity Assay Kit
Cat.No：
BC5460
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC5460-50T/24S Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Determination of Significance：

Phosphotransacetylase (PTA, EC 2.3.1.8) is one of the key enzymes related to acetate metabolism. Acetate kinase (ACK) and PTA catalyze acetic acid to generate acetyl coenzyme A, which is an important substance in the metabolism of carbohydrate, fat and protein.

Measurement Principle：

PTA catalyzes acetyl coenzyme A and phosphate to produce acetyl phosphate and coenzyme A, following the transformation from DTNB to yellow TNB. TNB has a characteristic absorption peak at 412 nm, and PTA activity is calculated by measuring the rate of increase in absorbance at 412nm.

Self Provided：

Spectrophotometer, water-bath, desk centrifuge, balance, mortar/homogenizer/cell ultrasonic crusher, transferpettor, 1 mL glass cuvette, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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