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Determination of Significance：
ATP (adenosine 5'-triphosphate) is found broadly in animals, plants, microorganisms and cultured cells, which is described as the energy currency in all living systems. Detecting the content of ATP and calculating the level of energy charge can reflect the state of energy metabolism.
Measurement Principle：
Hexokinase (HK) catalyzes the synthesis of glucose and ATP into 6-phosphate glucose. 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of glucose 6-phosphate and NADP to form NADPH. NADPH has a characteristic absorption peak at 340 nm, and the absorbance ratio of NADPH is in direct proportion to contents of ATP.
Self Provided：
Spectrophotometer/microplate reader, water bath/constant temperature incubator, refrigerated centrifuge, transferpettor, micro quartz cuvette/96 well UV flat-bottom plate, ice, mortar/ homogenizer, distilled water and chloroform.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
