English
中文
Product introduction：
Enzyme-labeled antibodies are specific, sensitive and safe immunochemical agents that are widely used in various branches of immunology, molecular biology and clinical medicine. At present, horseradish peroxidase (HRP) is commonly used to prepare HRP-antibody conjugate by labeling the IgG molecule with HRP after sodium iodate oxidation. All kinds of enzyme markers provided by Solarbio to users are labeled according to the modified sodium periodate oxidation method established by our company. A certain concentration of BSA has been added to each finished product as a stabilizer. The working concentration of the product varies according to the method. It is best to freeze at -20℃ before use to avoid repeated thawing.
Application:
antigenic determinant analysis and identification, a variety of antigens, antibodies quantitative, qualitative and localization analysis and determination.
Easy problems in the work:
1. The coated antibody or antigen is not comfortable, easy to produce a small positive value, or produce mutation, that is, the phenomenon of tube jumping.
2. If the antibody is coated with antiserum that is not purified, or too weakly purified, the positive value is small or there is no positive gradient.
3. Poor sealing or too concentrated marker can produce high contrast.
Main performance:
each 0.1ml liquid contains about 200μg/ each HRP, about 400μg/ each IgG, and 300μg/ each BSA.Stored at -20℃, stable for one year. It can be stored at 4 ° C for about 3 to 6 months, diluted 1:10, and can be used for about 30 days at 4 ° C. The diluent was as follows: pH7.4 PBST.
working titer:
Direct ELISA: 1:500-1:1000, using TMB as substrate (OD450nm)P/N = 2.5-3.0 as positive.
Immunoblotting: 1:200~400, immunohistochemistry: 1:20 ~40, dot blot: 1:200~400.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
