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Reactivity:Monkey
Application: WB,ELISA
Appearance:Liquid
Storage:Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Host:Rabbit
Product introduction：
Purified IgG antibodies with high purity (>95%) and high activity were prepared by ammonium sulfate salting out and DEAE- ion exchange cellulose chromatography or batch adsorption purification. All kinds of purified antibodies are provided as liquid, according to user requirements, with different concentrations, preferably stored at -20℃ before use, can be diluted at different concentrations according to the use method. Usually different purposes can be diluted with the following buffer solution, pay attention to complete defreezing, fully mixed. Before use, it is best to remove cold polymeric globulin and denatured protein by low temperature and high speed centrifugation, and re-quantification.
Gold labeling: Diluted with pH9.0 boric acid buffer, 1~2mg/ml labeling.
HRP labeling: diluted with 0.01mol/L pH9.0, CB or coated solution CB, labeled with 6~8mg/ml
Fluorescent labeling: Diluted with 0.5mol/L pH9.5 and CB(carbonate buffer), labeled with 10~20 mg/ml.
Biotin antibody: diluted with 0.1mol/L NaHCO3 and labeled with 1-2mg /ml. Other methods, such as immunodiffusion, can be diluted with 0.01mol/L pH7.2 PBS, and ELISA packets are treated with 10-20 μg/ml overnight at 4℃ or 37℃ for 2h.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
