Free Endotoxin Plasmid Extraction Maxi Kit
Cat.No:D1150 Solarbio
Storage:Enzyme Accessory store at -20℃;Endotoxin Scavenger store at 2-8℃;Other store at RT, 1 year.
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Molecular Biology >
Nucleic Acid Extraction/Purification >
Plasmid Extraction >
Free Endotoxin Plasmid Extraction Maxi KitStorage:Enzyme Accessory store at -20℃;Endotoxin Scavenger store at 2-8℃;Other store at RT, 1 year.
Qty:
Size:
Name | Free Endotoxin Plasmid Extraction Maxi Kit |
Storage | Enzyme Accessory store at -20℃;Endotoxin Scavenger store at 2-8℃;Other store at RT, 1 year. |
Unit | Kit |
Specification | 10T |
This kit uses alkaline cleavage method to lysate cells, and extracts plasmid DNA specifically according to the principle that the centrifuge adsorption column specifically binds DNA in solution under high salt state. The silicon matrix material used in the centrifugal adsorption column can efficiently and specifically adsorb DNA, which can maximize the removal of foreign proteins and other organic compounds in the cell. Endotoxin scavenger developed by Solarbio for maximum removal of endotoxins. 200-300μg plasmid DNA with high purity and high copy can be quickly extracted from 50-100mL E. coli LB medium, and the extraction rate can reach 85-90%. The plasmid DNA extracted with this kit is of high purity and can be used directly for demanding experiments such as cell transfection, as well as for a variety of other routine operations including enzyme digestion, PCR, sequencing, linking and transformation tests.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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