Yeast plasmid DNA was extracted by enzymic cracking of yeast cell wall and alkaline lysis of yeast cells. The yeast wall breaking enzyme can effectively destroy the yeast cell wall and increase the yield of yeast plasmid DNA. The silicon matrix material used in the adsorption column can efficiently and specifically adsorb DNA, which can maximize the removal of foreign proteins and other organic compounds in the cell. The yeast plasmid DNA extracted by this kit can be applied to a variety of conventional molecular biology experiments, including enzyme digestion, PCR, sequencing, linking and transformation tests. This kit is safe to operate without the use of toxic reagents such as phenol and chloroform.

Note:

1. Add RNaseA in solution YP1 before use (add all RNaseA provided in the kit), mix well, and store at 2-8℃.

2. Before the first use, anhydrous ethanol should be added to the bleach solution according to the instructions on the label of the reagent bottle to prepare the working solution (add 60mL anhydrous ethanol to the 15mL bleach solution).

3. Before use, please check whether solution YP2 and solution YP3 are turbidity. If turbidity exists, heat them in a water bath at 37℃ for several minutes, and then use them after the solution is recovered and clarified.

4, solution YP2, solution YP3 and bleaching solution should be closed immediately after use, if not specified, all centrifugal steps are to use table centrifuge at room temperature centrifuge.

5. Plasmid yield is related to yeast strain, plasmid copy number, culture conditions and other factors. Generally, the copy number of yeast plasmid is very low, so the plasmid yield is generally about 1μg per 5mL of bacterial solution extraction.

6, the volume of eluting buffer is better than 50μL, the volume is too small will affect the recovery efficiency; The pH of the eluent also affects the elution efficiency. If water is used as the eluent, ensure that its pH is around 8.0 (NaOH can adjust the pH of the water to this range). A pH below 7.0 will reduce the elution efficiency.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.