This kit uses a silicon substrate material that can bind DNA efficiently and specifically and a unique buffer system to recover DNA fragments from TAE or TBE agarose gels while removing impurities such as proteins, other organic compounds, inorganic salt ions and oligonucleotide primers. Fragments of 100bp - 10kb size can be recovered with a recovery rate greater than 80%(30-50% for<100bp and >10kb DNA fragments). The DNA recovered with this kit can be used for a variety of routine operations, including enzyme digestion, PCR, sequencing, library screening, linking and transformation experiments. Precautions: 1, it is best to use A new electrophoresis buffer during electrophoresis, so as not to affect the electrophoresis and recovery effect, the following step of the experimental requirements are high, should try to use the electrophoresis buffer. 2. When cutting glue, the ultraviolet irradiation time should be as short as possible to avoid damage to DNA. 3. When recovering<100bp and >10kb DNA fragments, the volume of the sol solution should be increased to extend the adsorption and elution time. 4, the recovery rate is related to the initial DNA amount and elution volume, the less the initial amount, the smaller the elution volume, the lower the recovery rate. 5. If not specified, all centrifuge steps are centrifuged at room temperature using a table centrifuge.

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