Reactive oxygen species detection kit (Reactive Oxygen Species Assay Kit) is a kind of kit for the detection of reactive oxygen species using fluorescent probe DCFH-DA. DCFH-DA itself has no fluorescence and can freely cross the cell membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to produce DCFH. However, DCFH cannot penetrate the cell membrane, so the probe can be easily loaded into the cell. Intracellular reactive oxygen species can oxidize non-fluorescent DCFH to produce fluorescent DCF. The level of intracellular reactive oxygen species can be known by detecting the fluorescence of DCF.

This kit provides reactive oxygen positive control reagent Rosup to facilitate the detection of reactive oxygen species. Rosup is a mixture with a concentration of 50 mg/mL.

The kit has the advantages of low background, high sensitivity, wide linear range and easy to use.

This kit can determine 100~500 samples.

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