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My CartReactivity：Mouse,Rat,Human
Application：ELISA,IHC,WB,FCM
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：8549
Host：Rabbit
Qty：
Size：
| Name | Anti-GPR49/LGR5 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000.IHC 1:100-500.FCM 1μg/Test.ELISA 1:5000-10000. |
| Target | GPR49/LGR5 |
| Background | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Swiss Prot | O75473 |
| Gene ID | 8549 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | A synthetic peptide of human GPR49/LGR5 |
| Public Immunogen Range | 211-310/907aa |
| Calculated MW | 98kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | ELISA FCM IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GPR49/LGR5 antibody diluted at 1:1000;Lane: Mouse Cerebrum Mouse Stomach Mouse Colon.
Immunohistochemistry of paraffin-embedded mouse brain tissue with GPR49 antibody diluted at 1:400.
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