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My Cart> Biochemical Assay Kits > Fatty Acid Metabolism Series > Hydroxymethylglutaryl Coenzyme A Synthase Activity Assay KitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Name | Hydroxymethylglutaryl Coenzyme A Synthase Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
The mevalonate pathway is a very important metabolic pathway involved in the synthesis of precursors of many important terpenoids and is an important pathway in terpenoid biosynthesis. Hydroxymethylglutaryl coenzyme A synthase catalyzes the formation of acetyl coenzyme A and acetoacetyl coenzyme A to produce hydroxymethylglutaryl coenzyme A, a key step in the biosynthesis of cholesterol and isoprenoids.
Measurement Principle：
Hydroxymethylglutaryl coenzyme A synthase catalyzes the formation of hydroxymethylglutaryl coenzyme A from acetyl coenzyme A and acetoacetyl coenzyme A. It also produces CoASH, which converts DTNB to TNB (yellow), with characteristic absorbance values at 412 nm.
Self Provided：
Spectrophotometer/ microplate Reader, low temperature centrifuge, adjustable transferpettor, water bath/ constant temperature incubator, micro glass cuvettes/96 well plates, mortar/homogenizer/cell ultrasonicator , ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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