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Your Position: Home > Molecular Biology > Nucleic Acid Extraction/Purification > Vacciniavirus-topoisomeraseI
Vacciniavirus-topoisomeraseI

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Vacciniavirus-topoisomeraseI
Cat.No：
R4030
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
R4030-500U Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

Product Introduction

The TopoisomeraseI is derived from vacciniavirus and was purified by gene recombination. In addition, it can recognize and cut the ends of double-stranded DNA [5 'C(T)CCTT↓], and form covalent links with DNA to form stable complexes. The covalent complex encounters the 5 '-OH group of DNA and reconnects to form a complete DNA strand. Therefore, the use of this enzyme can be used as an effective tool for DNA binding. It can be used for DNA carrier connection, notch repair, splice connection and other experiments.

Application

1. Dna-carrier connection

2. Notch repair

3. Connector connection

Precautions

(1) 1×vacciniaTOPOIBuffer: 50mMTris-acetate(pH7.5),

100mMNaCl,2.5mMMgCl2.

(2) The optimum reaction temperature of this enzyme is 37℃. The enzyme can be made at 80℃for 20min

Inactivation.

(3) When the enzyme cleaved coupling test, the 3 'of the [5' C(T)CCTT↓] site was identified.

The number of terminal bases affects the coupling efficiency.

(4) When the covalent complex is connected to the DNA molecule, the 5 '- end of the substrate needs to be protected

Leave the -OH group.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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