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Storage:Store at -20℃，3 years.
Product Introduction
The TopoisomeraseI is derived from vacciniavirus and was purified by gene recombination. In addition, it can recognize and cut the ends of double-stranded DNA [5 'C(T)CCTT↓], and form covalent links with DNA to form stable complexes. The covalent complex encounters the 5 '-OH group of DNA and reconnects to form a complete DNA strand. Therefore, the use of this enzyme can be used as an effective tool for DNA binding. It can be used for DNA carrier connection, notch repair, splice connection and other experiments.
Application
1. Dna-carrier connection
2. Notch repair
3. Connector connection
Precautions
(1) 1×vacciniaTOPOIBuffer: 50mMTris-acetate(pH7.5),
100mMNaCl,2.5mMMgCl2.
(2) The optimum reaction temperature of this enzyme is 37℃. The enzyme can be made at 80℃for 20min
Inactivation.
(3) When the enzyme cleaved coupling test, the 3 'of the [5' C(T)CCTT↓] site was identified.
The number of terminal bases affects the coupling efficiency.
(4) When the covalent complex is connected to the DNA molecule, the 5 '- end of the substrate needs to be protected
Leave the -OH group.
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