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Storage:Store at -20℃，3 years.
Product Introduction
The enzyme from BacillusstearothermophilusDNA
PolymeraseI, which was genetically engineered to remove its 5 '-3' exonuclide activity,
The 5 '-3' polymerase activity was retained. This enzyme has strong chain displacement and is therefore an excellent use of Isothermalamplification(LAMP,RT-LAMP). Compared with wild-type BstDNA polymerase (large fragment), this enzyme has significantly improved amplification speed, yield, salt tolerance and thermal stability, etc. At the same time, this enzyme can be amplified using dUTP as a substrate (large fragment Bst has no such activity).
Application
1.DNA isothermal amplification
2. Fast sequencing rich in GC structure
3. Rapid sequencing of trace template DNA
Precautions
(1) The used concentration of Mg2+ ranges from 4 to 10mM, and there is no Mg2+ in IsothermoBuffer. Generally, better LAMP results can be obtained under the condition of 6-8mMMg2+.
(2) It has been reported that adding TteUvrd helicase can improve the effect of LAMP.
(3) The specificity of amplification was tested using untemplated DNA as a control.
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