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Bst2.0 DNA Polymerase (containing glycerin)

Cat.No：PC2631 Solarbio

Storage：Store at -20℃，3 years.

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Product Information

Name	Bst2.0 DNA Polymerase (containing glycerin)
Storage	Store at -20℃，3 years.
Unit	Bottle
Specification	1600U

Product Introduction

The enzyme from BacillusstearothermophilusDNA

PolymeraseI, which was genetically engineered to remove its 5 '-3' exonuclide activity,

The 5 '-3' polymerase activity was retained. This enzyme has strong chain displacement and is therefore an excellent use of Isothermalamplification(LAMP,RT-LAMP). Compared with wild-type BstDNA polymerase (large fragment), this enzyme has significantly improved amplification speed, yield, salt tolerance and thermal stability, etc. At the same time, this enzyme can be amplified using dUTP as a substrate (large fragment Bst has no such activity).

Application

1.DNA isothermal amplification

2. Fast sequencing rich in GC structure

3. Rapid sequencing of trace template DNA

Precautions

(1) The used concentration of Mg2+ ranges from 4 to 10mM, and there is no Mg2+ in IsothermoBuffer. Generally, better LAMP results can be obtained under the condition of 6-8mMMg2+.

(2) It has been reported that adding TteUvrd helicase can improve the effect of LAMP.

(3) The specificity of amplification was tested using untemplated DNA as a control.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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