Endonuclease IV
Cat.No：Q3000 Solarbio
Storage：Store at -20℃，2 years.
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Molecular Cloning-Related Enzymes > PCR-Associated Enzyme > Endonuclease IV
Product Information
| Name | Endonuclease IV |
| Storage | Store at -20℃，2 years. |
| Unit | Bottle |
| Specification | 2KU |
Product Introduction
Endonuclease IV, also known as Nfo, is derived from E.coli and is involved in DNA damage repair. This enzyme can recognize the depurine/depyrimidine (AP) site on double-stranded DNA molecules, and cut the first phosphodiester bond at the 5′ end of the AP site to produce 3′ hydroxyl and 5′ deoxyribose phosphate ends. In addition, the enzyme also has 3′ diesterase activity, which can release phosphoglyceraldehyde, intact deoxyribose 5′ -phosphate and phosphoric acid from the 3′ terminal of DNA. In addition, the enzyme also has 3′-5′ exonuclide activity, and the progressive action of the enzyme on the substrate is affected by metal ions, DTT, EDTA, etc., and it is more inclined to act on the double chain of the 3′ concave end
DNA.
Application
1. Single cell gel electrophoresis
2.DNA damage and repair research
3.SNP Analysis
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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